Hepatitis C Virus Genotyping Based on 5′ Noncoding Sequence Analysis (Trugene)

, M . Bourliere , H . Khiri , V . de Noncoding Sequence Analysis ( Trugene ) ′ Hepatitis C Virus Genotyping

Clinical evaluation of two methods for genotyping hepatitis C virus based on analysis of the 5' noncoding region.

We compared the performance characteristics of a standardized direct sequencing method (TRUGENE HCV 5'NC; Visible Genetics Inc., Toronto, Ontario, Canada) and a reverse hybridization line probe assay (INNO-LiPA HCV II; Bayer Corp., Tarrytown, N.Y.) for genotyping of hepatitis C virus (HCV). Both methods are based on detection of sequence heterogeneity in the 5' noncoding (5'NC) region. Concorda...

hepatitis c virus ns5b sequence-based genotyping analysis of patients from the sharkia governorate, egypt

results the highest prevalent genotype was genotype 4a, which was found in 57 patients (92%) followed by 2 isolates (3%) with genotype 4o, 2 strains (3%) with genotype 1g and one isolate (2%) with genotype 4n. conclusions this molecular epidemiology study revealed high prevalence of hcv genotype 4, subtype 4a among egyptian patients residing in sharkia governorate, egypt. patients and methods t...

Sequence-based genotyping of hepatitis B virus in general popula-tion

    Background: Hepatitis B Virus (HBV) causes acute and chronic liver disease worldwide. HBV has eight genotypes (A to H) which is the reflection of its genome with their characteristic geographical distribution. Each genotype could have different pathogenic and therapeutic characteristics. There have been few records on HBV genotyping in general population from our region. This study aimed to...

Hepatitis C Virus NS5B Sequence-Based Genotyping Analysis of Patients From the Sharkia Governorate, Egypt

BACKGROUND Chronic hepatitis C virus infection and its sequela are major health problems facing the Egyptian community. The high prevalence and spread rates of the disease require serious actions to stop or decrease these rates. Determination of HCV genotypes and subgenotypes adds significant knowledge about the epidemiology of the disease, and provides an added value in the decision making pro...